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rat bbb culture kit  (PharmaCo Cell Company Ltd)

 
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    Structured Review

    PharmaCo Cell Company Ltd rat bbb culture kit
    (A) Schematic representation of an in vitro <t>BBB</t> model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic <t>plant-type</t> <t>ceramides</t> (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; * p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; * p <0.05 by t-test).
    Rat Bbb Culture Kit, supplied by PharmaCo Cell Company Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+bbb+culture+kit/pmc07605672-47-11-16?v=PharmaCo+Cell+Company+Ltd
    Average 90 stars, based on 1 article reviews
    rat bbb culture kit - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Blood-brain barrier permeability analysis of plant ceramides"

    Article Title: Blood-brain barrier permeability analysis of plant ceramides

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0241640

    (A) Schematic representation of an in vitro BBB model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic plant-type ceramides (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; * p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; * p <0.05 by t-test).
    Figure Legend Snippet: (A) Schematic representation of an in vitro BBB model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic plant-type ceramides (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; * p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; * p <0.05 by t-test).

    Techniques Used: In Vitro, Construct, Liquid Chromatography with Mass Spectroscopy



    Similar Products

    rat bbb culture kit  (PharmaCo Cell Company Ltd)
    90
    PharmaCo Cell Company Ltd rat bbb culture kit
    (A) Schematic representation of an in vitro <t>BBB</t> model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic <t>plant-type</t> <t>ceramides</t> (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; * p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; * p <0.05 by t-test).
    Rat Bbb Culture Kit, supplied by PharmaCo Cell Company Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+bbb+culture+kit/pmc07605672-47-11-16?v=PharmaCo+Cell+Company+Ltd
    Average 90 stars, based on 1 article reviews
    rat bbb culture kit - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic representation of an in vitro BBB model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic plant-type ceramides (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; * p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; * p <0.05 by t-test).

    Journal: PLoS ONE

    Article Title: Blood-brain barrier permeability analysis of plant ceramides

    doi: 10.1371/journal.pone.0241640

    Figure Lengend Snippet: (A) Schematic representation of an in vitro BBB model constructed from primary cultures of rat brain capillary endothelial cells, brain pericytes, and astrocytes. Synthetic plant-type ceramides (5 μM) were added to the medium in the inserts. After 30 min, conditioned medium in the bottom wells was collected and analyzed by LC-MS/MS. (B) Chemical structure of d18:2/C5-ceramide. (B) Chemical structures of d18:2/C6- and d18:2/C15-ceramides. (C) Levels of d18:2/C6-ceramide and d18:2/C15-ceramide. Data are presented as means ± SD (n = 3; * p <0.05 by t-test. (D) Levels of d18:2/C6-SM, d18:2/C15-SM, and GlcCer. Data are represented as means ± SD (n = 3; ns, not significant; * p <0.05 by t-test).

    Article Snippet: To evaluate the in vitro BBB permeability of plant ceramides, a Rat BBB Culture Model Kit (PharmaCo-Cell Co. Ltd., Nagasaki, Japan), which consist of primary cultures of brain capillary endothelial cells, brain pericytes and astrocytes [ , ], was used.

    Techniques: In Vitro, Construct, Liquid Chromatography with Mass Spectroscopy